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目的:探讨中国山东籍汉族大肠癌(CRC)患者人群中,IL-16基因多态性与大肠癌发病风险的关系。方法:在164例CRC患者和121名健康对照个体中,采用放大阻碍突变PCR(ARMS-PCR)方法对IL-16基因序列中的2个单核苷酸多态性(SNP)位点rs11556218和rs4778889进行基因分型,分析该2个位点的等位基因频率和基因型分布与CRC发病风险的关系。结果:在CRC患者中,rs11556218的T/G等位基因频率和基因型分布与对照组的差异有统计学意义(P=0.021 725,P=0.001 033);rs4778889的T/C等位基因频率及基因型分布与正常对照组的差异无统计学意义(P=0.057 946,P=0.064 229)。结论:IL-16的rs11556218基因多态性与可能与CRC的易感性增加有关。
Objective: To investigate the association between IL-16 gene polymorphism and the risk of developing colorectal cancer in Chinese Han population with colorectal cancer (CRC) in Shandong Province, China. METHODS: Two SNPs in the IL-16 gene sequence, rs11556218 and rs11556218, were amplified by ARMS-PCR in 164 CRC patients and 121 healthy controls. rs4778889 genotyping, analysis of the two loci allele frequency and genotype distribution and the relationship between the risk of CRC. Results: The frequency of T / G allele and genotype distribution of rs11556218 in CRC patients were significantly different from those in control group (P = 0.021 725, P = 0.001 033). The rs4778889 T / C allele frequency There was no significant difference in genotype distribution between the two groups (P = 0.057 946, P = 0.064 229). Conclusion: The rs11556218 polymorphism of IL-16 may be related to the increased susceptibility of CRC.