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目的:通过了解四种肺炎支原体检验方法,研究其临床应用价值,便于找出最佳的肺炎支原体检验方法。方法:对我院2013年3月至2013年6月收治的肺炎支原体患者进行抽样,选取126例经临床诊断的肺炎支原体感染者对比观察,取患者的咽拭子作培养和聚合酶链反应,取患者的血清作血清MP被动凝集法和免疫斑点方法检测,回顾上述四种检测结果与临床资料。结果:快速培养检验时间为二十四小时,其检测阳性率为43.65%(55/126);聚合酶链反应检验时间为八小时,其检测阳性率为61.9%(78/126);血清MP被动凝集法检验时间为四小时,其检测阳性率为85.71%(108/126);免疫斑点法检验时间为一小时,其检测阳性率为78.57%(99/126)。结论:在临床上诊断患者有无肺炎支原体感染,应根据患者病程、送检时间、检验方法的敏感性来选取最佳的检验方法。
Objective: To understand the four Mycoplasma pneumoniae test methods to study its clinical value, easy to find the best Mycoplasma pneumoniae test. Methods: Mycoplasma pneumoniae patients were selected from March 2013 to June 2013 in our hospital. 126 patients with mycoplasma pneumoniae who were clinically diagnosed were selected for comparison. Throat swabs were used for culture and polymerase chain reaction (PCR) Serum from patients with serum MP agglutination by passive agglutination and immune spots detection method, review the above four test results and clinical data. Results: The rapid culture test time was 24 hours, the positive rate was 43.65% (55/126); the PCR test time was eight hours, the positive rate was 61.9% (78/126); the serum MP Passive agglutination test for four hours, the positive rate was 85.71% (108/126); immune spot test for one hour, the positive rate was 78.57% (99/126). Conclusion: In clinical diagnosis of patients with or without Mycoplasma pneumoniae infection, the best test method should be selected according to the patient’s course of disease, delivery time, test method sensitivity.