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目的观察非甲状腺疾病综合征(nonthyroidal illness syndrome,NTIS)大鼠血清及脑组织甲状腺激素水平及其转运蛋白有机阴离子转运多肽(organic anion transporting polypeptide 1C1,OATP1C1)和单羧酸转运蛋白(monocarboxylatetransporter 8,MCT8)mRNA的变化。方法选取成年SPF级SD雄性大鼠23只,分为正常组(n=12)和NTIS组(n=11)。NTIS动物采用肝纤维化模型,利用四氯化碳(1∶6)用玉米油稀释,0.025 ml腹腔注射,3次/周,共8周。正常组大鼠相同剂量玉米油,腹腔注射,3次/周,共8周。造模成功后2组利用水迷宫法测试学习记忆能力。随后处死,取血及脑组织,放免法测定血清甲状腺激素(T3、T4、rT3、TSH)及脑组织甲状腺激素(T3、T4、rT3);RT-PCR法测定脑组织中OATP1C1及MCT8 mRNA水平。结果①NTIS组血清T3、T4水平较正常组明显降低[T3(0.53±0.18)mmol/L vs(0.73±0.22)mmol/L,P<0.05;T4(24.58±7.73)mmol/L vs(39.97±14.74)mmol/L,P<0.01],rT3、TSH水平2组之间无显著性差异(P>0.05);NTIS组脑组织中T3、T4浓度较正常组稍升高,但差异无统计学意义(P>0.05);②NTIS组OATP1C1和MCT8 mRNA水平较正常组明显升高[OATP1C1 mRNA(0.054±0.032)vs(0.019±0.016),P<0.01;MCT8 mRNA(0.058±0.048)vs(0.017±0.012),P<0.05];③2组大鼠空间探索和定位航行之间无显著性差异(P>0.05)。结论 NTIS大鼠脑内通过OATP1C1和MCT8代偿,保持脑内T3、T4水平相对稳定,从而维持中枢甲状腺功能正常。
Objective To observe the levels of serum thyroid hormone and its transporter protein of organic anion transporting polypeptide 1C1 (OATP1C1) and monocarboxylatetransporter 8 (NTA) in rats with nonthyroidal syndrome (NTIS) MCT8) mRNA changes. Methods Thirty male SD rats were divided into normal group (n = 12) and NTIS group (n = 11). NTIS animal model of liver fibrosis, carbon tetrachloride (1: 6) diluted with corn oil, 0.025 ml ip injection, 3 times / week, a total of 8 weeks. The same dose of corn oil in normal group was injected intraperitoneally 3 times a week for 8 weeks. After successful modeling, 2 groups used water maze to test learning and memory abilities. Serum thyroid hormones (T3, T4, rT3, TSH) and thyroid hormones (T3, T4, rT3) were determined by radioimmunoassay. The levels of OATP1C1 and MCT8 mRNA in brain tissue were determined by RT- . Results ① Serum levels of T3 and T4 in NTIS group were significantly lower than those in normal group [T3 (0.53 ± 0.18) mmol / L vs (0.73 ± 0.22) mmol / L, P <0.05; 14.74) mmol / L, P <0.01]. There was no significant difference between rT3 and TSH levels (P> 0.05). The concentrations of T3 and T4 in brain tissue of NTIS group were slightly higher than those in normal group (P> 0.05); ② The levels of OATP1C1 and MCT8 mRNA in NTIS group were significantly higher than those in normal group [OATP1C1 mRNA (0.054 ± 0.032) vs (0.019 ± 0.016), P <0.01; MCT8 mRNA (0.058 ± 0.048 vs 0.012), P <0.05]. There was no significant difference between the two groups in space exploration and navigation (P> 0.05). Conclusions NTIS rats are compensated by OATP1C1 and MCT8 in their brains to maintain the levels of T3 and T4 in the brain relatively stable, thus maintaining the normal function of the central thyroid.