[目的]明确莠去津降解菌对土壤污染的修复效果,并分析代谢过程。[方法]通过土壤修复试验,筛选莠去津污染修复的最优环境条件。利用高通量基因测序方法,分析莠去津的代谢过程。[结果]在接菌量为2%(v/v,OD600为1)、温度为25℃、时间为28 d时,修复效果最好。菌株对莠去津的降解是在6种酶及其辅助蛋白的共同作用下完成,生成5个中间产物。[结论]试验菌株具有较高的莠去津污染修复能力和独特的代谢过程。 [Objective] To clarify the remediation effect of atrazine-degrading bacteria on soil pollution and analyze the metabolic process. [Method] Through the soil remediation test, the optimal environmental conditions of atrazine remediation were screened. The metabolic process of atrazine was analyzed by high throughput gene sequencing. [Result] The best effect was achieved when the inoculation amount was 2% (v / v, OD600 was 1), the temperature was 25 ℃ and the time was 28 d. The degradation of atrazine by the strain was carried out by the combination of six kinds of enzymes and their accessory proteins, resulting in five intermediate products. [Conclusion] The test strains had high atrazine remediation ability and unique metabolic process.