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目的探讨阿托伐他汀对Krüppel样转录因子2(KLF2)在载脂蛋白E基因敲除小鼠中表达的影响。方法研究采用载脂蛋白E基因敲除小鼠,随机分为对照组和干预组,干预药物采用阿托伐他汀10mg/(kg·d),药物干预8周后分别用半定量RT-PCR,Westernblot,免疫组化检测主动脉KLF2的表达,HE染色切片计算斑块的相对面积。结果 KLF2在各个水平的表达干预组高于对照组[mRNA水平(0.85±0.01)比(0.48±0.02),蛋白水平(1.84±0.11)比(1.45±0.14);组织水平(0.40±0.06)比(0.26±0.09);均P<0.05];斑块相对面积:干预组(0.26±0.09)小于对照组(0.41±0.04)(P<0.01)。结论阿托伐他汀上调载脂蛋白E基因敲除小鼠颈动脉内KLF2表达。
Objective To investigate the effect of atorvastatin on the expression of Krüppel-like transcription factor 2 (KLF2) in apolipoprotein E knockout mice. Methods The apolipoprotein E gene knockout mice were randomly divided into control group and intervention group. Atorvastatin 10 mg / (kg · d) was used as the intervention drug. After 8 weeks of drug intervention, semi-quantitative RT-PCR, Western blot was used to detect the expression of KLF2 in aorta by immunohistochemistry. The relative area of plaque was calculated by HE staining. Results The level of KLF2 in intervention group was significantly higher than that in control group [mRNA level (0.85 ± 0.01) vs (0.48 ± 0.02), protein level (1.84 ± 0.11) vs (1.45 ± 0.14) (0.26 ± 0.09), all P <0.05]. The relative area of plaque was lower in intervention group (0.26 ± 0.09) than in control group (0.41 ± 0.04) (P <0.01). Conclusions Atorvastatin up-regulates the expression of KLF2 in the carotid artery of apolipoprotein E knockout mice.