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目的:探讨锌指蛋白A20(zinc finger protein A20)对D-半乳糖胺(D-galactosamine,D-GlaN)引起的急性肝损伤的保护作用。方法45只C57BL/6随机均分为三组:D-G1aN模型组(尾静脉注射D-G1aN 1.8 g/kg)、A20治疗组(尾静脉注射pCAGGS-A20质粒10μg/1 ml NS,8 h后注射D-G1aN 1.8 g/kg)、生理盐水对照组(尾静脉内注射生理盐水1 ml/只)。24 h后,断头取肝组织。ELISA法检测A20、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、干扰素-γ(interferon gamma,IFN-γ)蛋白表达水平。qPCR技术检测TNF-α、IFN-γmRNA表达水平。ELISA法检测肝组织内超氧化物岐化酶(superoxide-dimutase,SOD)、丙二醛(malonic dialdehyd,MDA)、髓过氧化物酶(myeloperoxidase ,MPO)活性的变化。应用HE染色技术观察肝病理损伤程度。结果D-G1aN组的A20蛋白质含量显著高于NS组,P<0.05,A20组的A20蛋白质含量又显著高于D-G1aN组与NS组,均P<0.05。A20组的TNF-α以及IFN-γmRNA和蛋白质表达水平均显著低于D-G1aN组,均P<0.05。A20组小鼠肝脏总SOD活性显著高于D-G1aN组,P<0.05,MDA、MPO活性显著低于D-G1aN组,均P<0.05。A20组小鼠肝组织病理学改变显著轻于D-G1aN组。结论A20具有抗氧化损伤的效应,A20转基因治疗能够有效抑制D-GalN引起的炎症反应。“,”Objective To explore the protective effect of zinc finger protein A20 on acute hepatic injury in mice induced by D-galactosamine. Methods Forty-five C57BL/6 mice were divided into three groups:D-galactosamine (D-GlaN) group injected with D-G1aN 1.8 g/kg through caudal vein, A20 group injected with pCAGGS-A20 plasmid 10μg/1 ml NS, and then D-G1aN 1.8 g/kg 8 h later through caudal vein, and NS group injected with NS 1 ml. Twenty-four hours later the mice were killed with their livers collected. The protein expression levels of A20, tumor necrosis factor-α(TNF-α), interferon-γ(IFN-γ) were detected by ELISA, the mRNA expression of TNF-αand IFN-γwere detected by reverse transcription polymerase chain reaction. The levels of superoxide-dimutase (SOD), malonic dialdehyd (MDA), and myeloperoxidase (MPO) were examined. The liver tissues underwent pathological examination with HE staining. Results The A20 protein level of the D-G1aN group was significantly higher than that of the NS group,P<0.05, and the A20 protein level of the A20 group was significantly higher than that of the D-G1aN group,P<0.05. The mRNA and protein expression level TNF-αand IFN-γin the liver tissue of the A20 group were both significantly lower than those of the D-GlaN group ,both P<0.05. The activity level of SOD in the liver tissue of the A20 group was significantly higher than that of the D-G1aN group,P<0.05, and the activity levels of MDA and MPO in the liver tissue of the A20 group were both lower than those of the D-G1aN group ,both P<0.05. The pathological changes in the liver tissue of the A20 group were significantly milder than those of the D-GlaN group. Conclusion A20 has the function against oxidative damage. A20 trans-gene therapy effectively inhibit the inflammatory response induced by D-G1aN .