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用甘油肌注法复制成大鼠急性肾功能衰竭（ＡＲＦ）模型，动态观察了肾脏功能与形态学变化，并用放免检测和免疫组化方法对ＡＲＦ大鼠肾组织中内皮素－１（ＥＴ－１）进行了定量与定位分析。结果显示，ＡＲＦ时血、尿和肾组织匀浆中ＥＴ－１含量增较正常值增高，免疫组化标记表明ＥＴ－１表达增强主要在肾小管上皮，且ＥＴ－１含量增长情况与ＡＲＦ肾组织学改变程度相一致，提示ＥＴ－１在ＡＲＦ发病机制中起重要作用。
Rat model of acute renal failure (ARF) was reproduced by intramuscular injection of glycerol, and the changes of renal function and morphology were observed dynamically. The expression of ET-1 in renal tissue of ARF rats was detected by radioimmunoassay (RIA) 1) Quantitative and positional analysis. The results showed that the levels of ET-1 in blood, urine and kidney homogenate increased more than normal at ARF. The expression of ET-1 was mainly in renal tubular epithelium with immunohistochemical staining. The increase of ET-1 level was correlated with ARF Histological changes consistent, suggesting that ET-1 plays an important role in the pathogenesis of ARF.