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用恒电位法在电化学聚合吡咯的同时，将酪氨酸酶固定在导电聚吡咯膜内，制成一种灵敏、稳定的酪氨酸酶电极。讨论了溶液ｐ Ｈ 值和聚合电位对酶固定化的影响，对酶分子嵌入前后吡咯膜的 Ｓ Ｅ Ｍ 图和 Ｃ Ｖ 曲线进行了分析和比较。该电极响应对甲苯酚的线性范围为５ ．０ ×１０ － ８ ～１ ．０ ×１０ － ６ ｍｏｌ· Ｌ－ １ ，最适ｐ Ｈ 值为６ ．６ ，酶反应表观上遵循 Ｍｉｃｈａｅｌｉｓ＿ Ｍｅｎｔｅｎ 动力学，表观米氏常数为２ ．２ ×１０ － ５ ｍｏｌ· Ｌ－ １ 。
With potentiostatic method in the electrochemical pyrrole at the same time, tyrosinase immobilized in the conductive polypyrrole membrane, made of a sensitive and stable tyrosinase electrode. The effects of pH value and polymerization potential on the immobilization of the enzyme were discussed. The S E M and C V curves of the pyrrole membrane before and after enzyme molecule embedding were analyzed and compared. The linearity of the electrode in response to p-cresol was 5. 0 × 10 - 8 ~ 1. 0 × 10 -6 mol · L -1, and the optimum p H value is 6. 6, The enzyme reaction apparently follows the Michaelis Menten kinetics, with an apparent Michaelis constant of 2. 2 × 10 - 5 mol · L -1.