以芦丁为对照品,AlCl3为显色剂,采用三波长-分光光度法测定普洱茶中总黄酮的含量。结果表明,黄酮质量浓度在3.92—39.2μg.mL-1范围内,分别在λ1=370nm、λ2=420nm、λ3=480nm处测定吸光度时,ΔA与浓度呈良好的线性关系,相关系数r=0.9998,平均回收率为100.15%,相对标准偏差为1.92%。方法的准确度与精密度均令人满意,而且操作简单。 Rutin was used as reference substance, AlCl3 was used as color reagent, and the content of total flavonoids in Pu-erh tea was determined by three-wavelength spectrophotometry. The results showed that there was a good linear relationship between ΔA and concentration when the concentration of flavonoids was in the range of 3.92-39.2μg.mL-1 and the absorbance was measured at λ1 = 370nm, λ2 = 420nm and λ3 = 480nm, respectively, and the correlation coefficient was 0.9998 , The average recovery was 100.15%, the relative standard deviation was 1.92%. The accuracy and precision of the method are satisfactory, and the operation is simple.