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以广东籼稻良种秋桂矮１１为材料，切取１１ｄ苗龄的叶基和叶鞘，游离原生质体；以台北３０９悬浮细胞为饲养层，放在饲养层顶部滤膜上培养，用Ｎ６＋２，４－Ｄ１．５ｍｇ．Ｌ－１、玉米素０．２ｍｇ．Ｌ－１、水解蛋白５００ｍｇ·Ｌ－１＋葡萄糖０．５ｍｏｌ·Ｌ－１＋Ｃ．１５％琼脂糖半凝固培养基。５－７ｄ后原生质体第一次分裂，秋桂矮１１分裂频率可达２７％：２５～３０ｄ形成小细胞团，逐步降低葡萄糖的含量，从０．５ｍｏｌ·Ｌ－１下降至０．２ｍｏｌ·Ｌ－１，促进细胞持续分裂，待愈伤组织达到１～２ｍｍ时，转入增殖和分化培养基；６０～７０ｄ后，秋桂矮１１获得再生植株。另一品种ＳＰ６０细胞团未见分化。
The leaves and leaf sheaths of 11-day-old seedlings and free protoplasts were obtained from the autumn indica rice variety Guiqian 11 in Taipei. The suspension cells of Taipei 309 were used as the feeder layer and cultured on the top membrane of the feeder layer with N6 + 2,4-D1. 5mg. L-1, zeatin 0.2mg. L-1, hydrolyzed protein 500 mg · L-1 + glucose 0.5 mol·L-1 + C. 15% agarose semi-coagulation medium. After 5-7 days, the protoplasts split for the first time, the split frequency of Qiu Gui short 11 was up to 27%. Small cell mass was formed from 25 to 30 days and the glucose content gradually decreased from 0.5 mol·L-1 to 0.2 mol·L-1 -1, to promote continuous cell division, to be callus reached 1 ~ 2mm, transferred to proliferation and differentiation medium; 60 ~ 70d, Qiu Gui short 11 regenerated plants. Another species of SP60 cell mass did not differentiate.