论文部分内容阅读
目的:研究DNA总体甲基化、羟甲基化水平与结直肠癌发生、发展及预后的关系。创新点:采用肿瘤与正常组织总体5-甲基胞嘧啶(5m C)、5-羟甲基胞嘧啶(5hm C)的比值并探讨其与结直肠癌临床病理变化和预后的关系。方法:采用酶联免疫吸附试验(ELISA)方法检测肿瘤与相应正常组织基因组中总体5hm C和5m C水平。结论:C/N-5m C比值的水平对结直肠癌的预后是一个独立的保护性因子(P=0.04)。当病人同时具有高水平的C/N-5hm C和低水平的C/N-5m C,其预后更差(P<0.01)。因此,可以使用5m C和5hm C对结直肠癌的发生和预后进行评估。
Objective: To study the relationship between the overall DNA methylation and methylation of DNA and the occurrence, development and prognosis of colorectal cancer. Innovative point: The ratio of total 5-methylcytosine (5m C) and 5-hydroxymethylcytosine (5hm C) in tumor and normal tissues was used to investigate the relationship between the 5-methylcytosine (5m C) and clinicopathological features and prognosis of colorectal cancer. Methods: Enzyme linked immunosorbent assay (ELISA) was used to detect the overall levels of 5hm C and 5m C in the tumor and corresponding normal tissues. CONCLUSIONS: The C / N-5m C ratio is an independent protective factor for the prognosis of colorectal cancer (P = 0.04). When the patient had both high levels of C / N-5hm C and low levels of C / N-5m C, the prognosis was worse (P <0.01). Therefore, the occurrence and prognosis of colorectal cancer can be assessed using 5 m C and 5 h C.