论文部分内容阅读
目的:研究结肠癌转移相关基因1(MACC1)在胃癌组织中的表达及其临床意义。方法:采用RT-qPCR检测胃癌组织株和67例胃癌组织及癌旁正常组织中MACC1mRNA的表达,免疫组化技术检测67例胃癌组织及癌旁正常组织中MACC1蛋白的表达,并分析其与临床病理资料的关系。结果:BGC823、SGC7901和MGC803 3种胃癌细胞株中MACC1mRNA的相对表达量分别为3.458 4±0.047 9、4.768 5±0.100 6和4.257 5±0.029 5,差异有统计学意义,F=295.131,P<0.05。癌组织MACC1mRNA表达量(5.560 0±2.853 1)较癌旁正常组织(2.190 1±1.675 9)显著增高,t=6.907,P=0.000。肿瘤组织中MACC1蛋白的阳性率为64.18%(43/67),显著高于癌旁正常组织5.97%(4/67),χ2=49.844,P<0.05,且MACC1蛋白的异常高表达与分化程度(χ2=14.492,P=0.000)、腹膜转移(χ2=4.251,P=0.039)、淋巴转移(χ2=11.978,P=0.001)、临床分期(χ2=11.024,P=0.001)密切相关,而与年龄(χ2=0.129,P=0.720)、性别(χ2=0.407,P=0.523)、肿瘤大小(χ2=0.017,P=0.897)、部位(χ2=3.021,P=0.082)及远处转移(χ2=0.000,P=1.000)无关。结论:MACC1可以作为预测腹膜转移、淋巴转移,评价胃癌进展的有效指标,可为胃癌患者的临床诊疗提供依据。
Objective: To study the expression of metastasis-associated gene 1 (MACC1) in gastric cancer and its clinical significance. Methods: The expression of MACC1 mRNA was detected by RT-qPCR in 67 gastric cancer tissues and adjacent normal tissues. The expression of MACC1 protein in 67 gastric cancer tissues and adjacent normal tissues was detected by immunohistochemistry, The relationship between pathological data. Results: The relative expression levels of MACC1 mRNA in BGC823, SGC7901 and MGC803 cell lines were 3.458 4 ± 0.047 9, 4.768 ± 0.100 6 and 4.257 5 ± 0.029 5, respectively, with a significant difference (F = 295.131, P < 0.05. The expression of MACC1mRNA in cancer tissues (5.560 ± 2.853 1) was significantly higher than that in normal tissues (2.190 ± 1.675 9), t = 6.907, P = 0.000. The positive rate of MACC1 protein in tumor tissues was 64.18% (43/67), significantly higher than that in normal adjacent tissues (5.97%, 4/67), χ2 = 49.844, P <0.05, and the abnormally high expression and differentiation of MACC1 protein (χ2 = 14.492, P = 0.000), peritoneal metastasis (χ2 = 4.251, P = 0.039) and lymph node metastasis (χ2 = 11.978, P = 0.001) (Χ2 = 0.127, P = 0.720), gender (χ2 = 0.407, P = 0.523), tumor size = 0.000, P = 1.000). Conclusion: MACC1 can be used as an effective index in predicting peritoneal metastasis and lymphatic metastasis, and evaluating the progress of gastric cancer. It can provide evidence for the clinical diagnosis and treatment of gastric cancer.