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按照一定的比例，在小牛血清的水溶液中加入丙酮和乙醇，然后在加热的情况下可形成稳定的小于５００ｎｍ的微球胶体溶液。这种方法产生的微球可在较低温度（７０～７５℃）和较短时间（２０分钟）内达到稳定，比较传统的高温（１００℃以上）和长时间（３０分钟以上）的加热制备法，这种丙酮乙醇加热变性技术有许多优点。这篇文章描述了制备过程的工艺条件，如丙酮、乙醇和小牛血清比例；加热时间和加热温度对微球形成以及对四氯四碘荧光素包裹率的影响。由本方法产生的微球可包裹４０％左右的四氯四碘荧光素。在３７℃和ＰＢＳ溶液中，上述微胶囊在１５天时间里释放了起始四氯四碘荧光素包含量的２５～６０％。
According to a certain proportion, acetone and ethanol are added to the aqueous solution of bovine serum, and then a stable colloidal solution of the microspheres less than 500 nm can be formed under heating. The microspheres produced by this method can be stable at lower temperature (70 ~ 75 ℃) and shorter time (20 minutes). Compared with traditional high temperature (above 100 ℃) and long time (more than 30 minutes) Act, this ethanol acetone heat denaturation has many advantages. This article describes the process conditions of the preparation process, such as the ratio of acetone, ethanol and calf serum; the effect of heating time and heating temperature on the formation of microspheres and the encapsulation efficiency of tetrachloro tetraiodofluorescein. The microspheres produced by this method can enclose about 40% tetrachloro tetraiodo-fluorescein. At 37 [deg.] C in PBS solution, the microcapsules released 25-60% of the initial tetrachlorotetraosine fluorescein content over a period of 15 days.