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用氧电极法测定了两个品种小麦线粒体有关呼吸功能的3项指标,研究其冻害问题。结果表明,-15℃低温促进了线粒体呼吸状态4耗氧量的增大,对呼吸控制率(呼吸状态3与呼吸状态4的比值)和磷氧比却有降低作用,NaCl更加剧了零下低温(-15℃)对线粒体的伤害。零上低温(2℃)锻炼有削弱或减缓线粒体冻害的作用。小麦品种之间对-15℃低温或NaCl的反应有差异。说明线粒体膜是冻害的位点之一,膜结构和功能是一致的,线粒体能量代谢失调可能是冻害的部分原因。
Oxygen electrode method was used to measure the respiratory index of three varieties of wheat mitochondria to study the frost damage. The results showed that hypothermia at -15 ℃ increased the oxygen consumption of mitochondrial respiration state 4 and decreased the respiratory control rate (the ratio of respiration state 3 to respiration state 4) and the P / (-15 ℃) on mitochondrial damage. Zero on the cold (2 ℃) exercise to weaken or mitigate the role of mitochondrial frost damage. There is a difference between wheat cultivars responding to low temperature of -15 ° C or NaCl. Mitochondrial membrane is one of the sites of frost damage, the membrane structure and function are consistent, mitochondrial energy metabolism disorders may be part of the cause of frost damage.