愈伤组织及其细胞的状态可通过调整培养基成分来调控。一般情况下，生长素、还原态氮、ＫＣｌ促进愈伤组织生长，细胞分裂素、硝态氮抑制愈伤组织生长。绝大多数易培养基因型诱导的愈伤组织往往表现为质地紧硬或外软内硬，不适于进行悬浮培养和原生质体培养。利用不同水平的２，４－Ｄ、ＮＨ＋４、谷氨酰胺、水解酪蛋白、ＫＣｌ、ＫＴ、６－ＢＡ、ＮＯ－３等进行处理，可使不适的愈伤组织变成适宜的类型，也可使那些生长过旺而不分化的愈伤组织分化出植株。用这些方法，从６个小麦基因型的原生质体中培养出了再生植株 The state of callus and its cells can be regulated by adjusting the composition of the medium. Under normal circumstances, auxin, reduced nitrogen, KCl promote callus growth, cytokinin, nitrate nitrogen inhibition of callus growth. Most of the easy-to-genotype-induced calluses tend to be harder in texture or soft in hard, not suitable for suspension culture and protoplast culture. Treatment with different levels of 2,4-D, NH + 4, glutamine, hydrolyzed casein, KCl, KT, 6-BA, NO-3 and the like can make unsuitable callus the appropriate type, Calluses that grew well without differentiation were differentiated from the plants. Using these methods, regenerated plants were grown from the protoplasts of 6 wheat genotypes