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30℃水相体系中“一锅法”快速制备固定化氯过氧化物酶(CPO@ZIF-8),在构筑金属有机沸石咪唑骨架结构(ZIF-8)的同时将氯过氧化物酶(CPO)固定在其三维纳米孔道中.温和的条件为固定化酶制备过程中酶活性的保持提供了前提.结构和性能表征说明酶分子的引入并不改变ZIF-8材料的孔道结构,同时酶分子在CPO@ZIF-8中呈现出在整体骨架材料中的嵌入式均匀分布.与先构筑ZIF-8骨架材料,然后通过表面吸附来固定酶分子的方法相比,通过将酶分子引入整体骨架材料中不仅提高了酶的固载量,更主要的是利用ZIF-8材料的高比表面积提高了固定化CPO的催化效率,同时基于三维孔道提供的刚性屏蔽环境有效改善了CPO在极端反应条件下的热稳定性、酸碱稳定性和对有机溶剂的耐受性.
(CPO @ ZIF-8) was prepared rapidly in the system of 30 ℃ water phase using the “One-pot method” method. While building up the metal organic zeolite imidazole framework structure (ZIF-8), chloroperoxide Enzyme (CPO) is immobilized in its three-dimensional nanopore.Mild conditions provide the prerequisite for the retention of enzyme activity during the preparation of immobilized enzyme.Characterization of structure and properties indicate that the introduction of enzyme molecules does not change the pore structure of ZIF-8 material, At the same time, the enzyme molecules showed a uniform embedded distribution in the whole framework material in CPO @ ZIF-8. Compared with the method of constructing the ZIF-8 framework material and then immobilizing enzyme molecules by surface adsorption, The overall framework material not only increases the enzyme immobilization capacity, but also mainly utilizes the high specific surface area of the ZIF-8 material to improve the catalytic efficiency of the immobilized CPO. Meanwhile, based on the rigid shielding environment provided by the three-dimensional pore channels, the CPO is effectively improved at the extreme Thermal stability, acid-base stability and resistance to organic solvents under reaction conditions.