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目的探讨苦瓜水提取物的抗突变作用。方法应用彗星实验及Ames实验对苦瓜水提取物进行抗突变研究。结果彗星试验2.5,5.0,10.0g/(kg.bw)3个剂量组与阳性对照组比较,差异有统计学意义(P<0.05),减少环磷酰胺(CP)引起的细胞DNA损伤;Ames试验5000,1667,556μg/皿3个剂量组在+/-S9的条件下,均能不同程度地抑制二氨基芴(2-AF)、敌克松(Dexon)引起的TA98、TA100回变菌落数的增加。结论在本实验条件下,苦瓜水提取物具有明显的抗突变作用。
Objective To investigate the antimutagenic effect of bitter gourd water extract. Methods The anti-mutation of bitter gourd water extracts was studied using comet assay and Ames test. Results The comet assay 2.5, 5.0, 10.0g/(kg.bw) 3 dose groups compared with the positive control group, the difference was statistically significant (P <0.05), reduce DNA damage caused by cyclophosphamide (CP); Ames Experiment 5000, 1667, 556μg/dish three dose groups in the +/-S9 conditions, can inhibit different levels of diaminoanthraquinone (2-AF), Dexon (Dexon) caused by TA98, TA100 reversion colonies The increase in the number. Conclusion Under the experimental conditions, the bitter gourd water extract has obvious antimutagenic effect.