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目的比较4种染色方法制作旋毛虫肌幼虫囊包标本的效果。方法 1醋酸卡红(含铁)染色液染色:染色1.5h,不分色;2固绿染色液染色:染色4s,不分色;3醋酸明矾卡红色液染色:染色30h,分色;4乙醇硼砂卡红染色液染色:染色22h,不分色。结果醋酸明矾卡红染色制作的标本囊包轮廓清晰,梭形显著,与周围组织分界明显,囊壁内、外层空隙明显,囊内幼虫清晰。结论醋酸明矾卡红染色制作的标本,染色效果好,易于观察。
Objective To compare the effect of four kinds of staining methods on the production of Trichinella spiralis larvae. Method 1 acetic acid card red (iron) stain staining: staining 1.5h, no color; 2 solid green stain staining: staining 4s, no color; 3 acetate red card staining solution: staining 30h, color separation; 4 Ethanol borax card red stain staining: staining 22h, regardless of color. Results Acetate alum card red staining of the sachet outline clear, significant spindle with the surrounding tissue obvious boundaries, the outer wall of the capsule, the outer gap clear lacunar cysts clear. Conclusion Acetate alum card red staining of specimens, dyeing effect is good, easy to observe.