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目的研究婴儿巨细胞病毒(CMV)肝炎淋巴细胞免疫功能变化与年龄分布特点。方法流式细胞术检测110例婴儿CMV肝炎患者及55例健康同龄儿外周血T淋巴细胞亚群(CD3/CD4/CD8)、B淋巴细胞(CD19)、NK细胞(CD16+56)的百分率,两组结果进行比较。并对婴儿CMV肝炎患者年龄进行统计。结果 CMV肝炎年龄1月婴儿占25%,2月婴儿占50%,3月婴儿占15%;巨细胞病毒肝炎与健康对照组相比,CD4+及CD4+/CD8+比值明显下降(P<0.01、P<0.001),CD8+细胞明显升高(P<0.001),较对照组差异有统计学意义,CD3+、CD19+及CD16+56+细胞结果两组间比较差异无统计学意义(P>0.05)。结论婴儿CMV肝炎感染与年龄相关,绝大多数在出生后1~3月,患儿细胞免疫功能受损,辅助性T细胞功能低下,抑制性T细胞功能亢进。CD4+、CD8+及CD4+/CD8+比值结果明显异常,T细胞亚群检测可以作为婴儿CMV肝炎的辅助诊断指标。婴幼儿出生后1~3月对巨细胞病毒感染的预防应得到足够重视。
Objective To study the changes of lymphocyte immune function and age distribution of infant cytomegalovirus (CMV) hepatitis. Methods The percentage of peripheral blood T lymphocyte subsets (CD3 / CD4 / CD8), B lymphocytes (CD19) and NK cells (CD16 + 56) in 110 infants with CMV hepatitis and 55 healthy infants were determined by flow cytometry. The two sets of results are compared. And the age of infants with CMV hepatitis statistics. Results The incidence of CMV hepatitis was 25% in January, 50% in February and 15% in March. Compared with healthy controls, the ratio of CD4 + and CD4 + / CD8 decreased significantly (P <0.01, P (P <0.001). There was significant difference between the two groups (P <0.001). There was no significant difference between the two groups in CD3 +, CD19 + and CD16 + 56 + cells (P> 0.05). Conclusion The infection of CMV in infants is age-related. Most of them have impaired cellular immune function, poor helper T cell function and inhibitory T cell function from January to March after birth. The results of CD4 +, CD8 + and CD4 + / CD8 + were significantly abnormal. The detection of T cell subsets could be used as a diagnostic indicator of infant CMV hepatitis. Infants from 1 March after birth on the prevention of cytomegalovirus infection should be given enough attention.