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目的探讨镉致雌性大鼠的生殖系统损害及生殖功能的影响。方法将雌性成年Sprague Dawley(SD)大鼠20只随机分成两组:对照组(C组)和氯化镉(CdCl_2)组(Cd组)。Cd组雌鼠腹腔注射1.0 mg/kg CdCl_2溶液,每天1次,每周5 d,连续6周;C组雌鼠在对应时间腹腔注射同量生理盐水。染毒第20天起每12 h用阴道脱落细胞涂片法连续观察并记录动情周期;6周给药结束后,两组大鼠麻醉后心脏取血,测定镉及相关生殖激素含量;剖取子宫及卵巢检测其中镉含量。结果与C组相比,Cd组雌鼠动情周期和动情间期异常率均明显升高(P<0.05);与C组相比,Cd组雌鼠血清镉、子宫镉及卵巢镉含量明显升高(P<0.01);血清中雌二醇(E2)、孕酮含量较对照组明显下降(P<0.01);血清中卵泡刺激素(FSH)、黄体生成素(LH)含量两组间比较,差异无统计学意义(P>0.05)。结论进一步明确了镉对大鼠的雌性性腺生殖毒性及影响机制,明确了子宫、卵巢为镉的生殖系统损害的靶器官。
Objective To investigate the effects of reproductive system damage and reproductive function in female rats induced by cadmium. Methods Twenty female Sprague Dawley rats were randomly divided into two groups: control group (C group) and CdCl 2 group (Cd group). Cd group mice were intraperitoneal injection of 1.0 mg / kg CdCl 2 solution once a day for 5 days a week for 6 weeks; group C females at the corresponding time intraperitoneal injection of the same amount of saline. Vaginal exfoliated cell smear method was used to observe and record the estrous cycle continuously every 12 hours from the 20th day after infection. After 6 weeks’ administration, the hearts of two groups were anesthetized and the cadmium and related reproductive hormones were determined. Detection of uterine and ovary cadmium content. Results Compared with group C, the estrous cycle and estrous cycle abnormalities in female rats were significantly increased (P <0.05). Compared with group C, cadmium, uterine cadmium and cadmium levels in cadmium group were significantly increased (P <0.01). The contents of E2 and progesterone in serum decreased significantly compared with the control group (P <0.01). The levels of serum FSH and LH in the two groups were significantly higher than those in the control group , The difference was not statistically significant (P> 0.05). Conclusions The mechanism of cadmium toxicity on gonad reproductive system in rats is further clarified, and the target organ of uterus and ovary damaged by the reproductive system of cadmium is clarified.