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目的:观察回药爱康方对小鼠肿瘤生长的影响及对突变型p53蛋白(p53mt)表达的影响。方法:将55只C57小鼠常规接种Lewis肺癌细胞,24h后随机分为荷瘤模型组、化疗组、回药低剂量组、回药低剂量加化疗组、回药高剂量加化疗组。治疗后观察各组小鼠的脾脏、胸腺、瘤体质量,免疫组化法检测各组p53mt的表达差异。结果:①除化疗组外,其余各组小鼠体质量与荷瘤模型组比较差异均有统计学意义(P<0.05);②回药高剂量加化疗组抑瘤率为71.00%,与荷瘤模型组比较,各组瘤质量差异有统计学意义(P<0.05);③回药高剂量加化疗组p53mt表达阳性率为36.36%,与模型组比较有统计学意义(P<0.01);④与化疗组比较,各组小鼠脾脏指数及胸腺指数差异均有统计学意义(P<0.01)。结论:回药协同化疗抑瘤效果及下调突变型p53的表达效果优于单纯化疗,且回药爱康方可以降低化疗后的免疫抑制,这可能是回族医药治疗肿瘤的新靶点。
Objective: To observe the effect of Yiyukang Recipe on tumor growth in mice and its effect on the expression of mutant p53 protein (p53mt). Methods: Fifty-five C57 mice were routinely inoculated with Lewis lung cancer cells and then randomly divided into tumor-bearing model group, chemotherapy group, low-dose drug group, low-dose chemotherapy group and high-dose chemotherapy group. After treatment, the spleen, thymus and tumor mass in each group were observed. The expression of p53mt in each group was detected by immunohistochemistry. Results: ① The body weight of mice in other groups were significantly different from that of tumor-bearing model group except chemotherapy group (P <0.05); ② The tumor inhibition rate of high dose plus chemotherapy group was 71.00% (P <0.05); ③The positive rate of p53mt expression in the high dose plus chemotherapy group was 36.36%, which was statistically significant compared with the model group (P <0.01); ④ Compared with chemotherapy group, the difference of spleen index and thymus index in each group was statistically significant (P <0.01). CONCLUSION: The antitumor effect of the drug-returning chemotherapy combined with chemotherapy and the down-regulation of the expression of mutant p53 are better than that of the chemotherapy alone. The medicine Ikangfang can reduce the immunosuppression after chemotherapy, which may be a new target for the treatment of cancer by the Hui nationality medicine.