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以苹果“平邑甜茶”(Malus hupehensis var.pingyiensis)为微嫁接砧木,“寒富”(Malus domesticacv.‘Hanfu’)为接穗,研究了附加不同浓度6-BA的培养基、接穗不同生理状态、接穗具有叶片数量、不同接穗/砧木组合对嫁接成活率和嫁接口愈合的影响。结果表明:最适于微嫁接的培养基为MS+IAA 0.3mg/L+BA 1.0mg/L,选用继代培养40d的接穗和砧木,并且接穗部分具有2片真叶时的嫁接成活率最高;用“平邑甜茶”作为砧木的嫁接成活率高(81.3%),嫁接口愈合最好。该研究为下一步的转基因sRNA信号在砧木与接穗间的传递运输研究奠定基础。
Malus hupehensis var. Pingyiensis was used as the micro-grafted rootstock and Malus domesticacv.’Hanfu ’as the scion. The medium supplemented with different concentrations of 6-BA was studied. Scion of different physiological states, the scion has the number of leaves, different combinations of scion / rootstock grafting survival rate and grafting healing. The results showed that the best medium for MS treatment was MS + IAA 0.3 mg / L and BA 1.0 mg / L, the scion and rootstock were selected for subculture 40 days, and the highest survival rate ; Grafting survival rate of “Pingyi sweet tea” as rootstock was high (81.3%), and the grafting interface was the best. This study laid the foundation for the further study on the transmission and transport of transgenic sRNA signals between rootstock and scion.