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目的同时重建SD大鼠膀胱的运动和感觉神经支配,建立和鉴定动物模型,为进一步研究排尿中枢重塑及机制奠定基础。方法 45只雌性SD大鼠分为对照组(10只),神经根切断组(15只)和神经根吻合组(20只)。神经根切断组大鼠,将腰椎(L)4以下双侧脊神经前后根全部切断;神经根吻合组在切断脊神经根后,将双侧L4神经前后根与骶椎(S)1相应神经根吻合;对照组不做手术处理。术后6个月,取各组大鼠分别行尿流动力学检测、神经根电刺激、神经吻合口甲苯胺蓝染色、盆神经节注射荧光金神经示踪染色和膀胱湿质量测量。结果神经根吻合组大鼠膀胱最大容量、残余尿量及膀胱湿质量均小于神经根切断组而大于对照组;神经根吻合组大鼠最大排尿压与对照组差异无统计学意义(P>0.05),但大于神经根切断组(P<0.05)。吻合神经根行电刺激可于膀胱检测到压力变化。神经吻合口甲苯胺蓝染色可见神经通过率达到54%。盆神经节内注射荧光金后,神经根吻合组可见L4脊髓节段双侧灰质荧光金染色,神经根切断组和对照组未在相应脊髓节段检测到荧光金染色。结论从形态和功能角度证明成功建立同时重建膀胱感觉及运动神经支配的大鼠动物模型,为进一步研究排尿中枢重塑及机制奠定基础。
OBJECTIVE To reconstruct the motor and sensory innervation of the bladder of SD rats at the same time, and to establish and identify animal models to lay a foundation for further study on urinary central remodeling and mechanism. Methods 45 female SD rats were divided into control group (n = 10), nerve root excision group (n = 15) and nerve root anastomosis group (n = 20). Rats in the nerve root excision group were cut off all the anterior and posterior roots of the bilateral spinal nerves below the lumbar vertebra (L) 4. The anterior and posterior roots of the bilateral L4 nerves and the corresponding nerve roots of the sacral (S) 1 were anastomosed The control group did not undergo surgical treatment. Six months after operation, the rats in each group were subjected to urodynamic tests, electrical nerve root stimulation, toluidine blue staining of the nerve anastomosis, fluorescent gold nerve tracing of pelvic ganglion and measurement of bladder wet mass. Results The maximal urinary bladder volume, residual urine volume and bladder wet weight of rats in nerve root anastomosis group were less than those in control group. The maximal voiding pressure in rats with nerve root anastomosis was not significantly different from that in control group (P> 0.05 ), But greater than the nerve root excision group (P <0.05). Anastomosis nerve root electrical stimulation can be detected in the bladder pressure changes. Nerve anastomotic toluidine blue staining showed nerve passage rate reached 54%. Fluorogold was injected into the pelvis ganglion, and bilateral gray matter fluorescence staining of the L4 spinal cord segment was observed in the root-mean-square nerve root ganglion. Fluorescent gold staining was not detected in the corresponding spinal cord segments of the nerve root excision group and the control group. Conclusions From the perspective of morphology and function, it is demonstrated that successful establishment of an animal model of bladder sensation and motor innervation at the same time will lay the foundation for further study of urinary central remodeling and mechanism.