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从三个县出血热流行区捕鼠444只,取血清与出血热病人血清作“对流”,黑线姬鼠409只中阳性81只,阳性率19.80%;在发病高峰季节的11、12月及次年1月份的阳性率分别为17.64%、22.61%和14.89%。根据出血热病人血清与黑线姬鼠血清作“对流”试验均出现一定量的沉淀线,该沉淀线经漂洗不退,可被蛋白染剂所染色,阳性鼠血清可与本病其他病人血清重现阳性,但与其它疾病病人血清及呼肠病毒抗体血清为阴性;而对照鼠血清全部阴性。吸收试验证实阳性鼠血清中抗原可被出血热病人血清中抗体吸收,进一步证明出血热病人血清与黑线姬鼠血清的“对流”试验是抗原抗体的特异性结合,对确定黑线姬鼠是出血热的传染源提供了实验依据。
444 mice were caught from the hemorrhagic fever endemic areas of three counties. Serum of the patients with hemorrhagic fever and hemorrhagic fever was taken as convection, and 81 of them were positive, with a positive rate of 19.80%. In the peak season of onset, 11 and 12 months And the positive rates of January and January were 17.64%, 22.61% and 14.89% respectively. According to hemorrhagic fever serum and Apodemus agrarius for “convection” test showed a certain amount of precipitation line, the sedimentation line is not refunded, can be stained with protein stain, the positive rat serum with other patients with this disease serum Recurrence of positive, but with other diseases, serum and reovirus seros were negative; while the control mice were all negative. Absorption test confirmed that the positive mouse serum antigens can be absorbed by the antibody in the serum of patients with hemorrhagic fever, hemorrhagic fever patients serum further confirmed Apodemus Serum “convection” test is the antigen-specific antibody binding, determination of Apodemus agrarius is The source of haemorrhagic fever provides experimental evidence.