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以2种人体病原细菌为靶标菌,采用琼脂块法从越南槐内生真菌中筛选拮抗菌株,以改良的微量肉汤稀释法测定其代谢产物的抗菌活性;根据形态和分子特征鉴定拮抗菌株的分类学地位。结果表明,菌株JXRPH-14和TRXY-9的活菌块对病原细菌的抑菌圈直径分别约等于或大于阳性对照;其代谢产物对病原细菌的最小抑菌浓度为20~40μg/m L,仅为阳性对照的20~40倍;初步鉴定这2株内生菌为不同的镰孢菌。菌株JXRPH-14和TRXY-9鉴定为不同的镰孢菌,对参试2种人体病原细菌均显示较强的抗菌活性,具有较大的开发应用潜力。
Two kinds of human pathogenic bacteria were used as target bacteria, and the antagonistic strains were screened out from the fungi of Huai-an-dong by using agar block method. The antibacterial activity of their metabolites was determined by the modified broth microdilution method. The antagonistic strains were identified according to their morphological and molecular characteristics Taxonomic status. The results showed that the antibacterial circle diameter of viable bacteria of strains JXRPH-14 and TRXY-9 were about equal to or greater than that of positive control respectively. The minimum inhibitory concentration (MIC) of the metabolites to pathogenic bacteria was 20-40μg / m L, Only 20 to 40 times the positive control; preliminary identification of these two strains of endophyte Fusarium different. The strains JXRPH-14 and TRXY-9 were identified as different Fusarium species, showing strong antibacterial activity against the two kinds of human pathogenic bacteria tested, which has great potential for development and application.