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目的了解哈尔滨市儿童流感流行特征,为制定儿童流感防治策略提供科学依据。方法采集儿童流感样病例的鼻咽拭子标本,采用MDCK细胞培养法分离流感病毒,微量血凝抑制(HI)实验进行型别鉴定,并对其流行病学及病原学监测结果进行分析。结果从2006年10月～2007年3月采集的185份儿童流感样病例的鼻咽拭中,分离到流感病毒32株,阳性率为17.30%,均为H1N1亚型。4～7岁年龄段的儿童流感病毒分离阳性率最高,为30.00%。儿童流感病毒分离高峰主要出现在12月份,阳性率高达39.47%。结论哈尔滨市2006-2007年度儿童流感流行优势株为H1N1亚型流感病毒,儿童流感流行主要集中在12月份。今后应加强儿童流感的预防与控制。
Objective To understand the epidemiological characteristics of children’s influenza in Harbin and to provide a scientific basis for formulating prevention and control strategies for children’s influenza. Methods Nasopharyngeal swab specimens were collected from children with influenza-like illness. Influenza virus was isolated by MDCK cell culture, and type identification was carried out by micro-hemagglutination inhibition (HI). The epidemiological and etiologic monitoring results were also analyzed. Results From the nasopharyngeal swabs of 185 children with influenza-like illness collected from October 2006 to March 2007, 32 influenza viruses were isolated, the positive rate was 17.30%, all of which were H1N1 subtypes. The positive rate of influenza virus in children aged 4 to 7 years was the highest, which was 30.00%. The peak of separation of children’s influenza virus mainly in December, the positive rate as high as 39.47%. Conclusion The prevalence of influenza among children in Harbin in 2006-2007 is H1N1 subtype influenza virus, and the prevalence of influenza in children is mainly concentrated in December. Future prevention and control of childhood flu should be strengthened.