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将刚逸出于扁卷螺的曼氏血吸虫(波多黎各株)尾蚴,浓集成500条/毫升,以不同剂量的钴~(60) 照射,在含有水化乳蛋白、青霉素、链霉素的 Earle 氏液内洗两次,并用针头将其头与尾分离后,在30℃下孵育1小时,搅动后静止3分钟,弃去浮悬的尾部,余下的体部用 Earle 氏液稀释成不同浓度。在经实验证明彼此无差异的 GBA/Lac株、CBA/H-T.株和 NIA 株小鼠体上进行免疫试验,各次均分为下列3组,并设同性别同年龄的小鼠作为对照,组问并作“t”测验.
The cercariae of Schistosoma mansoni (Puerto Rico) which had just escaped from the flat snail were concentrated to 500 pieces / ml and irradiated with various dosages of cobalt ~ (60). Earle was collected from eggs containing hydrated milk protein, penicillin and streptomycin After two separate injections, the heads were separated from the tail by a needle, incubated at 30 ° C for 1 hour, agitated for 3 minutes, and the suspended tail was discarded. The remaining body was diluted with Earle’s solution to different concentrations . The mice of GBA / Lac strain, CBA / HT strain and NIA strain which were proved to be indifferent to each other were tested for immunity. The animals were divided equally into the following three groups and mice of the same sex and age were used as controls. Group asked and made “t” test.