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目的获取人ＩＬ－１８ｃＤＮＡ克隆。方法用ＲＴ－ＰＣＲ方法从人外周血单个核细胞中获取人ＩＬ－１８ｃＤＮＡ，将其克隆至天然蛋白表达载体ＰＢＶ２２０中，并进一步亚克隆至融合蛋白表达载体ＰＧＥＸ－４Ｔ－３中，对其进行酶切分析及序列测定。结果测序结果表明克隆至ＰＢＶ２２０中的ＩＬ－１８ｃＤＮＡ包含成熟ＩＬ－１８蛋白的全部编码序列，酶切分析表明一约４７０ｂｐ的基因片段克隆至ＰＧＥＸ－４Ｔ－３中，与理论预计的一致。结论获得了编码人ＩＬ－１８成熟活性蛋白的ｃＤＮＡ克隆。
Objective To obtain human IL-18 cDNA clone. Methods Human IL-18 cDNA was obtained from human peripheral blood mononuclear cells by RT-PCR and cloned into the native protein expression vector PBV220. The recombinant plasmid was subcloned into the fusion protein expression vector PGEX-4T-3 Digestion analysis and sequencing. Results The sequencing results showed that the IL-18 cDNA cloned into PBV220 contained the complete coding sequence of mature IL-18 protein. The digestion analysis indicated that an approximately 470 bp gene fragment was cloned into PGEX-4T-3, consistent with the theoretical expectation. Conclusion The cDNA clone encoding human IL-18 mature active protein was obtained.