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大豆花叶病毒(SMV) 在大豆( Glycine max L.) 上引起严重病害。利用RT_PCR 扩增并克隆了SMV_ZK( 一个中国SMV 分离株) 基因组中全部蛋白质编码区的cDNA。通过对HC_PRO、NIb 和CP编码区进行序列测定与分析,发现SMV_ZK 与SMV_G2 高度同源,从而在分子水平上证明在我国大豆作物中存在SMV_G2 类似株系。将SMV_ZKcDNA克隆于细菌表达载体,获得并提纯了6 种cDNA 的表达产物。这项工作将为进一步研究SMV 基因组的功能奠定基础。
Soybean mosaic virus (SMV) causes serious disease in soybean (Glycine max L.). The cDNA of the entire protein coding region in the genome of SMV_ZK (a Chinese SMV isolate) was amplified and cloned by RT_PCR. By sequencing and analyzing the coding regions of HC_PRO, NIb and CP, SMV_ZK was found to be highly homologous to SMV_G2, which proved that SMV_G2-like lines were present in soybean plants at the molecular level. The SMV_ZK cDNA was cloned into a bacterial expression vector to obtain and purify the expression products of six kinds of cDNAs. This work will lay the foundation for further study on the function of SMV genome.