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解析骨干亲本产量性状分子模块对小麦品种分子设计与分子育种具有重要作用.以突破性高产小麦品种川麦42和川农16及其构建的127个重组自交系(RIL)群体(F10)为实验材料,分别于2014年和2015年在四川双流、什邡3个环境下种植,并测量千粒重、穗粒数等产量性状.利用Illumina公司的小麦90K SNP芯片对亲本和群体进行全基因组81 587个单核苷酸多态性分型.使用QTL Ici Mapping软件绘制遗传连锁图,并定位到一些控制产量性状的数量性状位点(QTL).结果显示:遗传图谱含8 580个SNP标记,全长3 147.1 c M,平均密度0.37 c M/标记.共定位到21个效应来自川麦42的产量性状QTL(解释表型变异>10%),分别位于1B、1D、2B、2D、3B、3D、4A、5A、5B、6B和7B,控制着株高、穗长、穗重、千粒重等性状,解释表型变异10.01%-23.07%.其中,1D、2B、5A、5B存在控制株高、籽粒面积、千粒重、穗长、穗重,并且在多个环境下均出现的QTL.此外,2B、5A、5B、7B上存在同时控制多个性状的QTL.这些研究结果可为深入了解骨干亲本川麦42产量性状遗传特性提供重要信息.
It is important to analyze the molecular design and molecular breeding of wheat cultivars by analyzing the molecular traits of backbone parents.With breakthrough and high-yield wheat cultivars Chuanmai 42 and Chuannong 16 and 127 recombinant inbred lines (RIL) The experimental materials were planted in Shuangliu and Shishu of Sichuan Province in 2014 and 2015 respectively and the yield traits such as 1000-grain weight and grain number per spike were measured.The Illumina 90K SNP chip was used to carry out 81 587 whole genomes Single nucleotide polymorphism (SNP) typing. QTL mapping software was used to map the QTLs and to locate QTLs controlling yield traits. The results showed that the genetic map contained 8 580 SNPs, 3 147.1 cM and average density of 0.37 cM / marker, and mapped 21 yield traits QTL (explained phenotypic variation> 10%) from Chuanmai 42 in 1B, 1D, 2B, 2D, 3B, 3D , 4A, 5A, 5B, 6B and 7B, which controlled the phenotypic variation of 10.01% -23.07%, which controlled plant height, spike length, panicle weight and 1000-grain weight etc. Among them, Grain area, grain weight, spike length, panicle weight, and in multiple environments QTL occur. Further, 2B, 5A, 5B, while controlling the presence of the QTL traits plurality 7B. These results can provide important information for understanding the genetic characteristics Yield 42 characters present Chuanmai pro backbone.