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耐旱性是干旱地区稳定和增加大麦产量的一个关键因素。鉴定出与耐旱性相关的功能基因,一方面可了解大麦的耐旱机理,同时还可以促进利用生物技术来改良大麦的耐旱性。在研究中,2个在耐旱性上具有明显差异的大麦品种Tadmor(耐旱)和WI2291(干旱敏感)被选作材料,采用22000个ESTs(基因表达序列标签)的Affymetrix大麦基因芯片Barley1来分析生殖生长期干旱胁迫下2个大麦材料的差异表达基因。研究结果表明,干旱胁迫下2个大麦材料中有77个共调节基因,其中部分基因已被报道过可能与抗旱性相关。这些基因中已有功能注释的基因按其生物学功能被分为14组,猜测它们是干旱胁迫的响应基因,在抗旱性上可能不起重要作用,或者是必需的但单独不足以提高大麦的抗旱性。进一步比较2个材料差异表达的基因,发现二材料之间有372个受干旱调节基因的差异。这些基因中有功能注释基因的生物学功能中可分为15组,其中一些已被认为与抗旱性相关;而对那些未知功能的基因,推测可能亦在大麦的抗旱性上扮演一定的角色。研究所得结果可为阐述生殖生长期大麦的耐旱性机理提供新的认识。
Drought tolerance is a key factor for stabilizing and increasing barley production in arid regions. Identification of functional genes related to drought tolerance, on the one hand to understand the mechanism of barley drought, but also can promote the use of biotechnology to improve barley drought tolerance. In this study, two barley cultivars Tadmor (drought tolerant) and WI2291 (drought sensitive) with significant differences in drought tolerance were selected as materials and 22,000 ESTs (gene expression sequence tags) of Barley1 from Affymetrix barley gene chip The differentially expressed genes of two barley cultivars under reproductive stage were analyzed. The results showed that there were 77 co-regulated genes in two barley materials under drought stress, some of which have been reported to be related to drought resistance. The genes that have been functionally annotated in these genes are grouped into 14 groups according to their biological function and it is hypothesized that they are response genes for drought stress that may not play an important role in drought resistance or may be necessary but not sufficient to increase barley Drought resistance. A further comparison of the two differentially expressed genes showed that there were 372 drought-regulated genes among the two materials. The biological functions of function annotated genes in these genes can be divided into 15 groups, some of which have been considered to be related to drought resistance; while those with unknown functions may also play a role in the drought resistance of barley. The results of this study may provide new insights into the mechanisms of drought tolerance in barley during reproductive growth.