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目的:优选南山茶花中总黄酮的提取工艺。方法:采用乙醇-水提取法从南山茶花中提取总黄酮,在单因素试验基础上,选取乙醇体积分数、提取时间、提取温度、料液比为考察因素,以总黄酮收率为评价指标,采用正交试验优选工艺。结果:总黄酮的最佳提取工艺为用20倍量的85%乙醇,95℃下提取2次,每次3h。在此条件下,总黄酮收率为1.52%。结论:优选的提取工艺合理、可行,可用于南山茶花中总黄酮的提取。
Objective: To optimize the extraction of total flavonoids from Camellia oleifera. Methods: Total flavonoids were extracted from Camellia oleifera by ethanol - water extraction method. Based on the single factor experiments, ethanol volume fraction, extraction time, extraction temperature and the ratio of solid to liquid were taken as the investigation factors. Total flavonoid yield was taken as the evaluation index, Orthogonal test using the preferred process. Results: The best extraction process of total flavonoids was 20 times the amount of 85% ethanol, extracted at 95 ℃ twice, each time 3h. Under these conditions, the yield of total flavonoids was 1.52%. Conclusion: The optimal extraction process is reasonable and feasible, which can be used for the extraction of total flavonoids from Camellia oleifera.