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目的应用家蚕核型多角体病毒载体，在家蚕幼虫中表达日本血吸虫中国大陆株23kDa膜蛋白基因。方法采用DNA重组技术，构建含Sj23基因的重组转移载体pBSj2。将此转移载体与经CvnⅠ酶切线形化的亲本病毒Bm－BacPAK6DNA通过脂质体法共转染BmN细胞，通过蓝白斑筛选，结合点杂交，纯化得到重组病毒。该重组病毒接种家蚕5龄幼虫，在家蚕中进行表达，用SDS－PAGE及Western－blot分析表达产物。结果Sj23基因在家蚕幼虫中成功地得到了表达，表达产物的分子量为23．7kDa，并具抗原性。结论该研究为发展基因工程血吸虫疫苗提供了一条新的途径。
Objective To express the 23 kDa membrane protein gene of Schistosoma japonicum in Chinese silkworm larvae using silkworm nuclear polyhedrosis virus vector. Methods Recombinant transfer vector pBSj2 containing Sj23 gene was constructed by DNA recombination technology. BmN cells were co-transfected with Bm-BacPAK6 DNA, a parental virus linearized by Cvn I, and then purified by lipofectamine. The fifth instar larvae of silkworm were inoculated with the recombinant virus and expressed in silkworms. The expressed products were analyzed by SDS-PAGE and Western-blot. Results The Sj23 gene was successfully expressed in silkworm larvae. The expressed product has a molecular weight of 23.7 kDa and is antigenic. Conclusion This study provides a new way to develop genetically engineered schistosome vaccine.