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目的:探讨阿普唑仑抗抑郁作用与脑内四氢孕酮含量变化的联系。方法:选用经典抑郁动物实验强迫游泳实验和悬尾实验。在大鼠强迫游泳实验和小鼠悬尾实验上,单次腹腔给予阿普唑仑(0.5,1和2 mg/kg)后,分别检测5 min内游泳不动时间和悬尾不动时间。在完成大鼠强迫性游泳后24 h内,酶联免疫吸附剂测定法检测其四氢孕酮含量。结果:阿普唑仑(1和2 mg/kg)在以上两个抑郁实验中可以显著缩短游泳不动时间(1 mg/kg:158 s和2 mg/kg:152 s)(P<0.05)和悬尾不动时间(1 mg/kg:133 s和2 mg/kg:121 s)(P<0.05)。酶联免疫吸附剂测定法显示,阿普唑仑(1和2 mg/kg)可提高前额皮层(1 mg/kg:125 pg/m L和2 mg/kg:150 pg/m L)(P<0.05),海马体(2 mg/kg:175 pg/m L)(P<0.05)和杏仁核(1 mg/kg:198 pg/m L和2 mg/kg:200 pg/m L)(P<0.05)四氢孕酮含量水平。结论:阿普唑仑在动物实验中具有显著抗抑郁作用,且该抗抑郁活性与前额皮层、海马体和杏仁核四氢孕酮含量提高有关。
Objective: To investigate the relationship between the antidepressant effect of alprazolam and the content of tetrahydrocanone in the brain. Methods: Forced swimming test and tail-hanging experiment were selected for classic depression animal experiment. In rats forced swimming test and mouse tail suspension test, after a single intraperitoneal administration of alprazolam (0.5, 1 and 2 mg / kg), the swimming immobility time and the tail suspension time were measured within 5 min. Within 24 h after completion of compulsive swimming in rats, the content of tetrahydroprotetone was measured by enzyme-linked immunosorbent assay. Results: Alprazolam (1 and 2 mg / kg) significantly reduced swimming immobility time (1 mg / kg: 158 s and 2 mg / kg: 152 s) in both depressive episodes (P <0.05) And tail-rest time (1 mg / kg: 133 s and 2 mg / kg: 121 s) (P <0.05). Enzyme-linked immunosorbent assay showed that alprazolam (1 and 2 mg / kg) increased prefrontal cortex (P <0.05) and P <0.05), hippocampal (2 mg / kg: 175 pg / m L) and amygdala (1 mg / kg: 198 pg / m L and 2 mg / kg: 200 pg / m L) P <0.05) tetrahydroprolone level. CONCLUSIONS: Alprazolam has significant antidepressant effects in animal experiments and this antidepressant activity is associated with increased progestin, hippocampal and amygdalone levels.