【摘 要】
：
目的 观察氢气能否减轻小鼠过敏性鼻炎.方法 BALB/c雌鼠24只,随机分成正常组、模型组、氮氧组、氢氧组.除正常组外,其余三组小鼠,前14 d内,每2天一次,腹腔注射溶有50μg卵白
【机 构】
：
中国人民解放军空军军医大学航空航天临床医学中心,西安 710032;中国人民解放军联勤保障部队第九○○医院 福建医科大学福总临床医学院 厦门大学附属东方医院,福州 350025;中国人民解放军西部战区
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目的 观察氢气能否减轻小鼠过敏性鼻炎.方法 BALB/c雌鼠24只,随机分成正常组、模型组、氮氧组、氢氧组.除正常组外,其余三组小鼠,前14 d内,每2天一次,腹腔注射溶有50μg卵白蛋白(ovalbumin,OVA)+2 mg氢氧化铝[Al(OH)3]的生理盐水0.3 mL;第15～21天给予两鼻腔内滴10μL内含200μg OVA生理盐水进行过敏性鼻炎造模.在造模过程中,氮氧组小鼠每天给予吸入氮氧混合气体(66.7％N2+33.3％O2)2 h;氢气组每天给予吸氢氧混合气体(66.7％H2+33.3％O2)2 h.从第15～21天,观察各组小鼠鼻腔给药后10 min内搔鼻子及打喷嚏次数,于第21天统一处死各组小鼠,行鼻腔病理组织切片苏木素-伊红(HE)染色,进行鼻黏膜内嗜酸性粒细胞计数.结果 造模后,模型组和氮氧组小鼠搔鼻子次数较正常组增多,差异有统计学意义(P<0.05),而氢氧组小鼠搔鼻子次数与正常组间差异无统计学意义.造模后,模型组小鼠打喷嚏次数和鼻腔黏膜嗜酸性粒细胞较正常组明显增多(P<0.05),而氢氧组小鼠打喷嚏次数和鼻腔黏膜嗜酸性粒细胞计数较模型组、氮氧组少,差异有统计学意义(P<0.05).结论 氢气对小鼠过敏性鼻炎有一定的减轻作用.
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