论文部分内容阅读
[目的]观察雷帕霉素(rapamycin)体外对肝癌HepG-2细胞生长抑制及侵袭能力的影响。[方法]用不同浓度的雷帕霉素作用于体外培养的HepG-2细胞,MTT法检测细胞胞生长抑制率;应用Matrigel胶模拟基质检测细胞侵袭能力。[结果]雷帕霉素能显著抑制HepG-2细胞的生长,呈现出明显的量-效关系和时-效关系。雷帕每素还能降低肿瘤细胞的侵袭能力,随剂量增高降低越明显。各药物处理组与阴性对照组相比差异有统计学意义(P﹤0.05)。[结论]雷帕霉素能有效地抑制肝癌细胞HepG-2的增殖,并且能降低细胞的侵袭能力。
[Objective] To observe the effect of rapamycin on the growth inhibition and invasion ability of HepG-2 cells in vitro. [Method] Different concentrations of rapamycin were used to effect HepG-2 cells cultured in vitro. The cell growth inhibition rate was measured by MTT assay. The invasion ability of cells was detected by Matrigel gel matrix. [Result] Rapamycin could significantly inhibit the growth of HepG-2 cells, showing a dose-effect relationship and time-effect relationship. Reipha also reduced the invasiveness of tumor cells, with the dose increased more significantly. Each drug treatment group and the negative control group, the difference was statistically significant (P <0.05). [Conclusion] Rapamycin can effectively inhibit the proliferation of HepG-2 cells and reduce the invasiveness of HepG-2 cells.