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[目的]明确山西省苹果树腐烂病主要致病菌Valsa mali对甲基硫菌灵、苯醚甲环唑和嘧菌酯3种常用防治药剂的敏感性,为苹果树腐烂病化学防治提供依据。[方法]采用菌落生长速率法测定了山西省8个苹果产区分离到的53个黑腐皮病菌V.mali菌株对3种杀菌剂的敏感性。[结果]3种杀菌剂EC_(50)范围依次为0.013~1.027 mg/L(均值为0.516 mg/L)、0.042~41.372 mg/L(均值为7.509mg/L)和0.003~0.309 mg/L(均值为0.035 mg/L)。[结论]频次正态分布敏感性聚类分析表明:V.mali菌株群体对甲基硫菌灵的EC_(50)呈近正态分布,以EC_(50)均值作为甲基硫菌灵的相对敏感基线,未检测到甲基硫菌灵抗性菌株;V.mali菌株出现了对嘧菌酯和苯醚甲环唑敏感性降低的亚群体。敏感性聚类分析结果表明:山西省苹果树腐烂病菌株对药剂的敏感性无地域性差异。交互抗性分析结果表明:3种药剂两两间无交互抗性。
[Objective] The research aimed to determine the sensitivity of Valsa mali, a major pathogen of apple tree rot disease in Shanxi province, to three common control agents, thiophanate-methyl, difenoconazole and azoxystrobin, to provide the basis for chemical control of apple tree rot disease . [Method] The sensitivity of 53 strains of V.mali isolated from 8 apple producing areas in Shanxi Province to 3 fungicides was determined by colony growth rate method. [Results] The range of EC 50 of the three fungicides was 0.013-1.027 mg / L (mean 0.516 mg / L), 0.042-41.372 mg / L (mean 7.509 mg / L) and 0.003-0.309 mg / L (Mean 0.035 mg / L). [Conclusion] The results of frequency-normal distribution sensitivity cluster analysis showed that EC 50 of V.mali strain population was almost normal distribution to thiophanate-methyl, and the EC 50 mean value was the relative value of thiophanate-methyl Sensitive baseline, did not detect thiophanate-methyl resistant strains; V.mali strains appeared susceptible to azoxystrobin and difenoconazole sub-population. Sensitivity cluster analysis showed that there was no regional difference in the sensitivity of the apple tree rot disease isolates to the pesticides in Shanxi Province. The results of cross-resistance analysis showed that there was no cross-resistance among the three drugs.