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作者应用抗ＨＣＶＮＳ３区Ｃ３３ｃ抗原２Ｂ６株单克隆抗体和抗ＨＢｘＡｇ多克隆抗体，采用ＡＢＣ法对１０２例人原发性肝细胞肝癌（ＰＨＣ）组织进行了ＨＣＶ及ＨＢＶ抗原定位研究。ＨＣＶＣ３。抗原及ＨＢｘＡｇ在ＰＨＣ中的阳性检出率分别是８１．４％及７４．５％，Ｃ３３ｃ抗原或ＨＢｘＡｇ阳性占所检病例９４．１％，相同病例二者同时阳性为６１．８％。１０２例ＰＨＣ中５０例有癌旁肝组织，其Ｃ３３ｃ抗原和ＨＢｘＡｇ的阳性检出率分别是６２％和９２％。ＨＣＶＣ３３ｃ抗原定位于肝癌细胞的胞浆内，胞核未见阳性信号。Ｃ３３ｃ抗原阳性细胞在ＰＨＣ中呈散在、局灶分布为主，在癌旁肝组织呈弥漫分布为主。本文结果提示ＨＣＶ感染在ＰＨＣ的发生中可能起重要作用。
Using anti-HCV NS3 C33c antigen 2B6 strain monoclonal antibody and anti-HBxAg polyclonal antibody, the authors used ABC method to study the localization of HCV and HBV antigen in 102 cases of human primary hepatocellular carcinoma (PHC). HCVC3. The positive detection rates of antigen and HBxAg in PHC were 81.4% and 74.5%, respectively. C33c antigen or HBxAg positive accounted for 94.1% of the cases. The same cases were both positive at 61.8%. Among the 102 cases of PHC, 50 cases had paraneoplastic liver tissue, and the positive detection rates of C33c antigen and HBxAg were 62% and 92%, respectively. The HCVC33c antigen was localized in the cytoplasm of hepatoma cells and no positive signal was found in the nucleus. The C33c antigen-positive cells were scattered and localized mainly in PHC, and diffusely distributed in the adjacent liver tissue. The results of this study suggest that HCV infection may play an important role in the development of PHC.