以底物浓度、pH、温度、培养时间作为处理,采用靛酚蓝比色法测定灰棕紫泥土脲酶动力学参数和反应条件。结果表明,灰棕紫泥土脲酶酶促反应V0与[S]的关系呈二次线性关系,[S]为11.67%时,V0达极大值;Km值在中性、35℃环境下较小,Vmax值在中性环境下较大,与温度呈二次正相关;脲酶酶促反应最适pH值为6.9、最适培养时间为24h、最适底物浓度为10%、最适温度为45℃。 The substrate concentration, pH, temperature and incubation time were taken as the treatments. The kinetic parameters and reaction conditions of Ureasem were determined by indophenol blue colorimetry. The results showed that there was a quadratic linear relationship between V0 and [S] in the urease enzymatic reaction, and V0 reached a maximum at [S] of 11.67%. The Km value was relatively low at 35 ℃ , The value of Vmax was larger in neutral environment and quadratic positive correlation with temperature; the optimum pH value of urease enzymatic reaction was 6.9, the optimum incubation time was 24h, the optimum substrate concentration was 10%, the optimum temperature was 45 ° C.