观察临床69例疑难肿瘤病例,采用ABC法,使用10种抗体(Keratin,CEA,S-100,Vimentin,Lysozyme,a_1-AT,a_1-ACT,Myoglobin,Thyroglobulin,LCA等)做免疫组化染色研究。结果:阳性者64例(92.8％),阴性者5例(7.2％).并对横纹肌肉瘤、滑膜肉瘤、恶性纤维组织细胞瘤和肺未分化癌等肿瘤病理形态学特点、鉴别诊断及组织发生来源进行了讨论;还探讨了免疫组化技术检查存在的问题。 Observed 69 cases of clinically intractable tumor cases, using ABC method, using 10 antibodies (Keratin, CEA, S-100, Vimentin, Lysozyme, a_1-AT, a_1-ACT, Myoglobin, Thyroglobulin, LCA, etc.) for immunohistochemical staining . Results: 64 cases (92.8%) were positive, and 5 cases (7.2%) were negative. The pathological features, differential diagnosis, and organization of tumors such as rhabdomyosarcoma, synovial sarcoma, malignant fibrous histiocytoma, and undifferentiated lung cancer were analyzed. The source of the occurrence was discussed; the problems with immunohistochemical techniques were also examined.