目的:研究不同的方法对’过山香’胚性悬浮细胞原生质体分离的影响,筛选最适合用于’过山香’香蕉胚性悬浮细胞原生质体分离的方案。方法:用不同浓度、不同组合的酶液对’过山香’原生质体进行分离,并对酶液的甘露醇含量、pH值进行调节。结果:3.0%纤维素酶R-10+0.2%果胶酶Y-23的是最佳酶组合;酶解8h、酶液中含0.41M甘露醇、酶液pH值为5.3时,获得原生质体产量最高。结论:合适的酶组合、酶解时间、酶液的渗透压和pH值对’过山香’香蕉胚性悬浮细胞原生质体的分离有明显的促进作用。 OBJECTIVE: To study the effects of different methods on the separation of protoplasts of ’Shannon’ ’s embryogenic suspension cells, and to screen for the most suitable protoplast isolation method for’ Shannon ’Banana Embryogenic Suspension Cells. Methods: The protoplasts of ’Pulsatilla chinensis’ were separated by enzyme solution of different concentrations and different combinations, and the mannitol content and pH value of the enzyme solution were adjusted. Results: The optimal enzyme combination was 3.0% Cellulase R-10 + 0.2% Pectinase Y-23. After enzymolysis for 8h, the enzyme solution contained 0.41M mannitol and the pH value of the enzyme solution was 5.3, The highest yield Conclusion: Appropriate enzyme combination, enzymolysis time, osmotic pressure and pH value of the enzyme solution can significantly promote the isolation of protoplasts from the suspension culture of ’Shannon’ banana embryos.