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目的比较不同分子生物学方法检测手足口病标本的敏感性及应用价值。方法选取山东莘县疾病预防控制中心2012年1月~2013年8月收集的100例手足口病标本为研究对象,根据分子生物学检测方法不同,将其分为巢式PCR组、一步法RT-PCR(逆转录-聚合酶链反应)组及实时RT-PCR组,对3组肠道病毒71型检测阳性率结果进行对比分析。结果巢式PCR对住院标本、轻症标本及重死标本检测的EV 71阳性率分别为84.62%、25%及83.33%,实时RT-PCR检测的80.77%、23.31%及77.78%,均明显高于一步法RT-PCR检测的15.38%、8.92%、44.44%,比较有统计学意义(P<0.05)。结论手足口病原体主要是EV71及CA16,且巢式PCR和实时RT-PCR生物检测敏感性相对一步法RT-PCR要高。
Objective To compare the sensitivity and the value of different molecular biological methods in the detection of hand, foot and mouth disease specimens. Methods A total of 100 hand-foot-mouth disease specimens collected from January 2012 to August 2013 in Shandong Xinxian Center for Disease Control and Prevention were selected for the study. According to the different methods of molecular biology, they were divided into nested PCR group, one-step RT -PCR (Reverse Transcription-Polymerase Chain Reaction) group and real-time RT-PCR group. The results of positive detection rate of three groups of enterovirus 71 were compared and analyzed. Results The positive rates of EV 71 detected by nested PCR in hospital specimens, mild cases and severely dead specimens were 84.62%, 25% and 83.33%, respectively, and 80.77%, 23.31% and 77.78% in real-time RT- The one-step RT-PCR detection of 15.38%, 8.92%, 44.44%, more statistically significant (P <0.05). Conclusion The pathogens of hand-foot-mouth are mainly EV71 and CA16, and the sensitivity of nested PCR and real-time RT-PCR bioassay is higher than that of one-step RT-PCR.