论文部分内容阅读
用组织化学和生化方法探讨了幼龄大鼠内毒素血症脾急性非特异性免疫反应。腹腔注入内毒素（ｉ．ｐ．ＬＰＳ）１ｈ，脾内碱性磷酸酶（ＡＬＰａｓｅ）阳性细胞开始增多，至１２ｈ红髓和边缘区内呈现大量的ＡＬＰ阳性细胞，２４～７２ｈ这些阳性细胞逐渐减少至正常。电镜下证实这些阳性细胞多数为单核－巨噬细胞和中性粒细胞。腹注ＬＰＳ６～１２ｈ，脾索和淋巴小结周围呈现较多的一氧化氮合成酶（ＮＯＳ）阳性细胞。腹注ＬＰＳ６～１２ｈ生化方法测定脾匀浆ＮＯＳ和ＮＯ的水平显示两者均升高。本研究的结论是：（１）ＬＰＳ可刺激脾内单核－巨噬细胞和中性粒细胞增多，表明它们有明显的动态分布；（２）脾脏ＮＯＳ活性反应和ＮＯＳ及ＮＯ含量有严格的时间性；（３）就幼龄大鼠来说，腹注ＬＰＳ的有效毒性只能维持在２４ｈ之内。
The acute and nonspecific immune response of spleen in young rats with endotoxemia was explored by histochemical and biochemical methods. Intraperitoneal injection of endotoxin (i.p.LPS) 1h, spleen alkaline phosphatase (ALPase) positive cells began to increase, to 12h red pulp and marginal zone showed a large number of ALP positive cells, 24 ~ 72h these positive cells decreased To normal. Electron microscopy confirmed that the majority of these positive cells were monocytes - macrophages and neutrophils. Abdominal injection of LPS6 ~ 12h, splenic cord and lymph nodules showed more nitric oxide synthase (NOS) positive cells. Abdominal injection of LPS6 ~ 12h biochemical methods to determine the level of NOS and NO in the spleen homogenate showed that both were elevated. The conclusions of this study are: (1) LPS can stimulate splenic monocytes - macrophages and neutrophils increased, indicating that they have a significant dynamic distribution; (2) spleen NOS activity and NOS and NO content of the strict Timeliness; (3) In young rats, the effective toxicity of peritoneal LPS can only be maintained within 24h.