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目的:研究何首乌总苷(polygonum multiflorum total glycoside,PMTG)对载脂蛋白E基因缺陷(ApoE-/-)小鼠实验性动脉粥样硬化(atherosclerosis,AS)病变形成及细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)与血管细胞黏附分子-1(vascular cell adhesion molecule-1,VCAM-1)的表达影响,探讨何首乌总苷抑制AS病变形成的可能机制。方法:将ApoE-/-小鼠随机分为4组:何首乌总苷高剂量组(150 mg.kg-1.d-1)、低剂量组(25 mg.kg-1.d-1)、阿托伐他汀阳性药物组(5 mg.kg-1.d-1)及模型对照组。给药第10周后全部处死,检测4组血清脂质含量,一氧化氮(NO),总抗氧化能力(TAOC),丙二醛(MDA),主动脉AS粥样斑块面积及其与管腔面积的比值,主动脉壁ICAM-1及VCAM-1表达。结果:何首乌总苷高、低剂量组及阳性药物组与模型对照组ApoE-/-小鼠比较,显示:①何首乌总苷高、低剂量可以显著降低血清总胆固醇(TC),甘油三酯(TG)水平(P<0.01),升高高密度脂蛋白(HDL)水平(P<0.01);②明显增加血浆NO及TAC(P<0.05及P<0.01),减少MDA的生成(P<0.01);③何首乌总苷可减轻AS病变,减小斑块面积与管腔面积比值(P<0.05);④何首乌总苷可下调ICAM-1及VCAM-1的表达(P<0.01)。结论:何首乌总苷可能通过调节ApoE-/-小鼠血脂代谢、增强其抗氧化能力及下调主动脉壁ICAM-1及VCAM-1表达等上述环节的共同作用与影响,阻止ApoE-/-小鼠实验性动脉粥样硬化病变形成的发生、发展。
Objective: To study the effect of Polygonum multiflorum total glycoside (PMTG) on atherosclerosis (AS) lesion formation and intercellular adhesion molecule-1 in apoE-/- mice ( Intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) expression affect the possible mechanism of total glucosides of Polygonum multiflorum on inhibiting the formation of AS lesions. Methods: ApoE-/- mice were randomly divided into 4 groups: high dose group of total glycosides of Polygonum multiflorum (150 mg.kg-1.d-1) and low dose group (25 mg.kg-1.d-1). Atorvastatin positive drug group (5 mg.kg-1.d-1) and model control group. After 10 weeks of administration, all rats were sacrificed and serum lipid content, nitric oxide (NO), total antioxidant capacity (TAOC), malondialdehyde (MDA), plaque area of aortic atherosclerosis (AS), and their levels were measured. The ratio of the lumen area, the expression of ICAM-1 and VCAM-1 in the aortic wall. Results: Compared with the model control group ApoE-/- mice, the total and high doses of total glucosides of Polygonum multiflorum showed that:1. High and low doses of total glycosides of Polygonum multiflorum can significantly reduce serum total cholesterol (TC) and triglyceride ( TG level (P<0.01) increased high-density lipoprotein (HDL) levels (P<0.01); 2 significantly increased plasma NO and TAC (P<0.05 and P<0.01) and decreased MDA production (P<0.01) ); 3 Polygonum aglycone can reduce AS lesions, reduce plaque area and lumen area ratio (P <0.05); 4 Polygonum glycosides of Polygonum multiflorum can downregulate the expression of ICAM-1 and VCAM-1 (P <0.01). Conclusion: The total glycosides of Polygonum multiflorum Thunb may prevent the ApoE-/- small by regulating the lipid metabolism in ApoE-/-mice, enhancing its antioxidative capacity and down-regulating the expression of ICAM-1 and VCAM-1 in the aortic wall. The occurrence and development of experimental atherosclerotic lesions in rats.