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目的观察感染家兔在治疗前后血清中循环抗原和循环抗体的动态变化情况。方法采用日本血吸虫4株单克隆抗体3D8，5C7，SM21－3及SM38m（靶抗原分别为肠相关多糖蛋白抗原、肠相关蛋白抗原、虫卵抗原和成虫表膜抗原）作为捕获抗体，用斑点酶联免疫吸附试验（Dot－ELISA）检测4类循环抗原，与常规ELISA检测IgG抗体比较。结果感染日本血吸虫家兔在经硝唑咪治疗后1～2个月，循环抗原基本转阴，而IgG抗体一直维持较高水平。结论循环抗原具有较好的疗效考核价值，IgG抗体不具有疗效考核作用。
Objective To observe the dynamic changes of circulating antigens and circulating antibodies in infected rabbits before and after treatment. Methods Four monoclonal antibodies against Schistosoma japonicum, 3D8, 5C7, SM21-3 and SM38m (the target antigens were gut-associated proteoglycan, enteroprotein antigen and adult epidermal antigen respectively) were used as capture antibody. The four kinds of circulating antigens were detected by the method of Dot-ELISA, which was compared with that of routine ELISA. Results Infection of Schistosoma japonicum rabbits 1 to 2 months after treatment with nitrazepam, circulating antigens basically turned negative while IgG antibodies remained at a high level. Conclusion The circulating antigen has a good therapeutic value, IgG antibody does not have the effect of evaluation.