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目的:观察丹参酮ⅡA磺酸钠(STS)对膀胱出口部分梗阻(PBOO)大鼠膀胱中a-SMA表达的影响,探究其可能的作用机制。方法:通过膀胱颈部分结扎法建立大鼠PBOO模型,24只SD大鼠随机分为PBOO组、STS治疗组(STS组)以及假手术组(Sham组),每组8只。STS组每天给予STS腹腔注射(10 mg/kg),持续4周。PBOO组及Sham组给予等量生理盐水腹腔注射。应用透射电镜及Masson染色检测膀胱组织中胶原纤维的表达,免疫组化和RT-PCR检测膀胱中a-SMA的蛋白及mRNA的表达,应用Western blot检测膀胱组织中ERK1/2、P-ERK1/2蛋白的表达。结果:PBOO组胶原纤维表达量高于Sham组(P<0.05),STS组胶原纤维表达量低于PBOO组(P<0.05)。PBOO组a-SMA蛋白及mRNA表达高于Sham组(P<0.05),STS组低于PBOO组(P<0.05)。三组ERK1/2蛋白表达差异无统计学意义(P>0.05),P-ERK1/2蛋白的表达,PBOO组高于Sham组,STS组低于PBOO组(P<0.05)。结论:STS能够抑制PBOO大鼠a-SMA的表达,其机制可能是通过抑制MAPK通路中ERK1/2蛋白的活化实现的。
Objective: To observe the effect of sodium tanshinone Ⅱ A sulfonate (STS) on the expression of a-SMA in the bladder of bladder outlet obstruction (PBOO) rats and to explore its possible mechanism. Methods: PBOO model was established by partial ligation of bladder neck. 24 SD rats were randomly divided into PBOO group, STS treatment group (STS group) and sham operation group (Sham group), with 8 rats in each group. The STS group was given STS intraperitoneally (10 mg / kg) daily for 4 weeks. PBOO group and Sham group were given the same amount of saline intraperitoneal injection. The expression of a-SMA protein and mRNA in the bladder was detected by immunohistochemistry and RT-PCR. The expressions of ERK1 / 2 and P-ERK1 / 2 in bladder tissues were detected by Western blot. 2 protein expression. Results: The expression of collagen fibers in PBOO group was higher than that in Sham group (P <0.05). The expression of collagen fibers in STS group was lower than that in PBOO group (P <0.05). The expression of a-SMA protein and mRNA in PBOO group was higher than that in Sham group (P <0.05), but lower in STS group than in PBOO group (P <0.05). The expression of ERK1 / 2 protein in the three groups was not significantly different (P> 0.05). The expression of P-ERK1 / 2 was higher in PBOO group than in Sham group and lower in STS group than in PBOO group (P <0.05). CONCLUSIONS: STS can inhibit the expression of a-SMA in PBOO rats by inhibiting the activation of ERK1 / 2 protein in MAPK pathway.