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目的:应用非浓缩尿蛋白电泳技术,分析肾脏疾病患者蛋白尿成分的特点。方法:采用全自动电泳仪以十二烷基磺酸钠-琼脂糖凝胶电泳法(SDS-AGE)对45例肾脏疾病患者的尿标本进行尿蛋白电泳分析。结果:45例肾脏疾病患者的尿标本经尿蛋白电泳后,根据蛋白质的分子量的大小顺序排列在凝胶上可区分为非选择性肾小球性、选择性肾小球性、肾小管性及混合性蛋白尿。结论:琼脂糖尿蛋白电泳技术简便快速、无创伤,且实验结果与肾脏病理损害部位及程度有关,在肾脏疾病早期诊断有重要的意义。
Objective: To analyze the characteristics of proteinuria in patients with kidney diseases by using non-concentrated urine protein electrophoresis. Methods: Urine samples of 45 patients with renal diseases were analyzed by urinary protein electrophoresis with sodium dodecyl sulfate-agarose gel electrophoresis (SDS-AGE) using a fully automatic electrophoresis system. Results: Urine specimens of 45 patients with renal diseases were classified into non-selective glomeruli, selective glomeruli, renal tubules and glomeruli by urinary protein electrophoresis according to the order of their molecular weight Mixed proteinuria. Conclusion: Agar proteinuria electrophoresis technique is simple, rapid and noninvasive, and the experimental results are related to the location and degree of renal pathological damage. It is of great significance in the early diagnosis of renal diseases.