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目的：研究杂交瘤细胞的体外高密度培养，大量制备高纯度单克隆抗体（ＭｃＡｂ）．方法：应用Ｃｅｌｉ－ｇｅｎ５Ｌ细胞培养罐，以半连续灌注方式在１％的低血清条件下培养分泌抗疱疹病毒（ＨＳＶ）ＭｃＡｂ的杂交瘤细胞１Ａ１２．结果：细胞密度最高达１．７×１０７／ｍｌ，鼠ＩｇＧ最高为３５０μｇ／ｍｌ，连续培养２３ｄ，共获鼠ＩｇＧ５．３ｇ，平均滴度１∶６４．结论：半连续式培养杂交瘤细胞，细胞密度和抗体产量均优于纯批式培养．
OBJECTIVE: To study the in vitro high-density culture of hybridoma cells and to prepare high-purity monoclonal antibodies (McAbs) in large quantities. METHODS: Celi-gen5L cells were used to culture 1A12 cells secreting anti-herpesvirus (HSV) McAb under the condition of 1% low serum with semi-continuous perfusion. Results: The highest cell density was 1.7 × 107 / ml and the highest was 350μg / ml. After cultured for 23 days, the mouse IgG5.3g was obtained with the average titer 1:64. Conclusion: The semi-continuous culture of hybridoma cells, cell density and antibody yield were better than the pure batch culture.