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在厌氧条件下．用LKB－2107Batch微量热系统研究了在298．15K,0．1mol／L磷酸盐缓冲溶液（pH7．4）中谈树漆酶与5种废物的反应．测定了各反应的反应焓变△rHm，米氏常数Km，酶底复合过程的吉布斯自由能上△Go,复合物中电子转移速率常数k_2，以及漆酶对各种废物的催化活性EA讨论了在相同的实验条件下，各种酶底复合物的稳定性，底物官能团及其位置对酶反应中的电子转移速率和非酶反应中自由基聚合形式及产物结构的影响．
Under anaerobic conditions. The LKB-2107Batch micro calorimeter system was used to study the reaction of laccase and five wastes in a 0.1 mol / L phosphate buffer solution (pH7.4) at 298.15K. The reaction enthalpy change △ rHm, Michaelis constant Km, Gibbs free energy △ Go at the end of the enzyme complex, the electron transfer constant k 2 in the complex, and the catalytic activity of laccase on various wastes EA Under the same experimental conditions, the stability of various substrate complexes, the functional groups of the substrates and their positions on the electron transfer rate in the enzymatic reaction and the influence of the free radical polymerization and the structure of the product on the non-enzymatic reaction were discussed.