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目的：检测猴病毒40（SV40）DNA在人脑膜瘤中的定位表达情况，探讨其在脑膜瘤发生发展过程中的生物学意义。方法：采用地高辛标记探针原位杂交技术。结果：SV40DNA定位于脑膜瘤细胞核，阳性细胞呈弥漫性或局灶性分布；SV40DNA阳性率35．9％（37／103），正常脑组织均未检测出SV40DNA；SV40DNA阳性率与病理分级无关（P＜0．05）。结论：SV40感染与人脑膜瘤病因学密切相关；地高辛标记探针原住杂交技术，是探讨SV40病毒核酸在组织中定位、分布和感染机理的一种新的特异性方法。
OBJECTIVE: To detect the localization of SV40 DNA in human meningiomas and to explore its biological significance in the development of meningioma. Methods: Digoxigenin labeled probe in situ hybridization. Results: SV40DNA was located in the nucleus of meningioma, with diffuse or focal distribution of positive cells. The positive rate of SV40DNA was 35.9% (37/103), while no SV40DNA was detected in normal brain tissue. The positive rate of SV40DNA was not related to the pathological grade P <0.05). CONCLUSIONS: SV40 infection is closely related to the etiology of human meningiomas. The digoxigenin-labeled probe of native hybridization is a new and specific method to investigate the localization, distribution and infection mechanism of SV40 in nucleic acid.